anhydrous glacial acetic acid_Ingestion of higher concentrations causes immediate burning of the mouth and throat, breathing difficulty, drooling, difficulty swallowing, stomach pain and vomiting (there may be blood in the vomit).

Despite its advantages, the use of CRO3 in glacial acetic acid raises certain safety and environmental concerns. Chromium(VI) compounds are recognized as carcinogenic, highlighting the importance of proper handling and disposal practices in laboratory settings. It is imperative that chemists utilize personal protective equipment and adhere to stringent safety protocols when working with such substances. Moreover, the environmental impact of chromium waste necessitates the development of greener alternatives and methodologies in organic synthesis.

While glacial acetic acid is beneficial in many ways, it should be handled with care due to its corrosive nature. Proper laboratory safety procedures should be observed, including the use of gloves, goggles, and working in a well-ventilated area. Careful attention should also be paid to the concentration of acetic acid used, as excessive amounts can alter the properties of the gel and negatively affect electrophoresis results.